Journal: Biosensors
Article Title: Functional Evaluation and Nephrotoxicity Assessment of Human Renal Proximal Tubule Cells on a Chip
doi: 10.3390/bios12090718
Figure Lengend Snippet: hRPTECs model on the integrated biomimetic array chip (iBAC). ( A ) Picture of an integrated biomimetic array chip containing 24 units. ( B ) Schematic exploded view of a unit on the iBAC. ( C ) Diagram showing the hRPTECs cultured on the iBAC. ( D , E ) Immunofluorescence micrographs of the hRPTECs and HK2 cells cultured on the iBAC for 4 days labeled with acetylated tubulin (apical), villin (apical) and Na/K-ATPase (basolateral), (bar, 100 µm). ( F ) The coverage rate of the acetylated tubulin, villin and Na/K-ATPase on the hRPTECs and HK2 cells cultured on the iBAC on day 4. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: In this study, we constructed a renal proximal tubule model on an integrated biomimetic array chip (iBAC) driven by bidirectional shaking, and systematically compared the cell growth, barrier function, transporter expression and drug toxicity of the hRPTECs on the iBAC and the static Transwell.
Techniques: Cell Culture, Immunofluorescence, Labeling